Direct identification of Photobacterium damselae subspecies piscicida by PCR-RFLP analysis
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چکیده
منابع مشابه
Simple and rapid detection of Photobacterium damselae ssp. piscicida by a PCR technique and plating method.
AIMS To detect Photobacterium damselae ssp. piscicida using the PCR technique and plating method. METHODS AND RESULTS Two strains of P. damselae ssp. piscicida were isolated from cultured cobia (Rachycentron canadum) at two different fish farms in Taiwan. A pair of primers was designed to detect the capsular polysaccharide gene of P. damselae ssp. piscicida by PCR. Reference strains of differ...
متن کاملSuperoxide dismutase and catalase activities in Photobacterium damselae ssp. piscicida.
The ability of a set of Photobacterium damselae ssp. piscicida strains isolated from different fish species to produce different superoxide dismutase (SOD) and catalase enzymes was determined. Unlike other bacterial pathogens, P. damselae ssp. piscicida is not able to produce different isoforms of SOD or catalase containing different metal cofactors when cultured under oxidative stress induced ...
متن کاملPhenotypic characterization of the marine pathogen Photobacterium damselae subsp. piscicida.
The taxonomic position of Photobacterium damselae subsp. piscicida, the causative agent of fish pasteurellosis, is controversial as this organism has also been described as 'Pasteurella piscicida'. To clarify the taxonomic position of the pathogen, a total of 113 P. damselae subsp. piscicida strains and 20 P. damselae subsp. damselae strains, isolated from different geographical areas and from ...
متن کاملPhotobacterium damselae subsp. piscicida: an integrated view of a bacterial fish pathogen.
Pasteurellosis, or pseudotuberculosis, is a bacterial septicaemia caused by the halophilic bacterium Photobacterium damselae subsp. piscicida (formerly Pasteurella piscicida). Although this disease was first described in wild populations of white perch and striped bass, currently the natural hosts of the pathogen are a wide variety of marine fish. The disease has great economic impact both in J...
متن کاملIn vivo morphological and antigenic characteristics of Photobacterium damselae subsp. piscicida
The present study was conducted to examine the morphology and antigenicity of Photobacterium damselae subsp. piscicida by culturing the bacterium in vivo in the peritoneal cavity of sea bass (Dicentrarchus labrax) within dialysis bags with either a low molecular weight (LMW) cut-off of 25 kDa or a high molecular weight (HMW) cut-off of 300 kDa. Differences were observed in the growth rate betwe...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 2005
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao065053